Published in
Taylor and Francis Group, Bioscience, Biotechnology and Biochemistry, 1(70), p. 269-272, 2006
DOI: 10.1271/bbb.70.269
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Identification, Cloning, and Expression of theScytalidium acidophilumXYL1 Gene Encoding for an Acidophilic Xylanase
Journal article published in 2006 by
Bassam Al Balaa,
Johan Wouters,
Sophie Dogne 
,
Carlos Rossini,
Jean-Michel Schaus,
Eric Depiereux,
Jean Vandenhaute,
Isabelle Housen
,
Carlos Rossini,
Jean-Michel Schaus,
Eric Depiereux,
Jean Vandenhaute,
Isabelle Housen
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Abstract
We cloned XYL1, a Scytalidium acidophilum gene encoding for an acidophilic family 11 xylanase. The XYL1p protein was expressed in Pichia pastoris using the pPICZalphaA expression plasmid. The secreted protein was purified by TAXI affinity column chromatography. The purified XYL1p showed an optimum activity at pH 3.2 and 56 degrees C. The Michaelis-Menten constants were determined.