Insect societies are characterized by a relatively sophisticated division of labor; they form tightly knit groups that must effectively exclude non-members from the colony. However, the Neotropical predatory ant Ectatomma tuberculatum can harbor several specific myrmecophiles and, in particular, various eucharitid parasitoid wasp species. Adult wasps eclose in the host nests and are removed by worker ants without harm. Previous observations suggest that only a few workers perform this task. To test this hypothesis, we introduced different types of intruders, live and dead, pentane-washed broad-nosed grain weevil individuals (Caulophilus oryzae) and dead, pentane-washed eucharitids (Dilocantha lachaudii), into laboratory colonies containing individually marked workers. We recorded all encounters and behaviors until the intruders were removed. Certain workers removed intruders more frequently than expected by chance. The number of encounters with an intruder was positively correlated with the number of removals performed by the workers. For each nest, a small group of workers was identified as specialized in intruder removal. A subset of very committed workers in the specialists group that performed up to 57% of removals qualified as hyperspecialists or elite workers. The behavioral sequences differed based on the type and condition of the intruder: the sequence was more complex and included numerous aggressive behaviors (mandibular strikes and attempts at stinging) when workers encountered a live weevil. In contrast, the behavioral sequences with dead, pentane-washed insects were characterized by numerous detections through contact that did not lead to rejection and by the intruders simply being seized and removed from the nest. Overall, the data show that the ants discriminated between live and pentane-washed intruders and adjusted their behavior accordingly. This is the first demonstration in ants of both behavioral specialization and hyperspecialization in intruder removal.