Published in

American Society for Microbiology, Antimicrobial Agents and Chemotherapy, 5(46), p. 1535-1539, 2002

DOI: 10.1128/aac.46.5.1535-1539.2002

Links

Tools

Export citation

Search in Google Scholar

Chromosomal System for Studying AmpC-Mediated β-Lactam Resistance Mutation in Escherichia coli

Journal article published in 2002 by Joseph F. Petrosino, Amanda R. Pendleton, Joel H. Weiner, Susan M. Rosenberg ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

Full text: Download

Green circle
Preprint: archiving allowed
Upload
Green circle
Postprint: archiving allowed
Upload
Orange circle
Published version: archiving restricted
Upload
Policy details
Data provided by SHERPA/RoMEO

Abstract

ABSTRACT In some enterobacterial pathogens, but not in Escherichia coli , loss-of-function mutations in the ampD gene are a common route to β-lactam antibiotic resistance. We constructed an assay system for studying mechanism(s) of enterobacterial ampD mutation using the well-developed genetics of E. coli . We integrated the Enterobacter ampRC genes into the E. coli chromosome. These cells acquire spontaneous recombination- and SOS response-independent β-lactam resistance mutations in ampD . This chromosomal system is useful for studying mutation mechanisms that promote antibiotic resistance.