American Society for Microbiology, Antimicrobial Agents and Chemotherapy, 5(46), p. 1535-1539, 2002
DOI: 10.1128/aac.46.5.1535-1539.2002
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ABSTRACT In some enterobacterial pathogens, but not in Escherichia coli , loss-of-function mutations in the ampD gene are a common route to β-lactam antibiotic resistance. We constructed an assay system for studying mechanism(s) of enterobacterial ampD mutation using the well-developed genetics of E. coli . We integrated the Enterobacter ampRC genes into the E. coli chromosome. These cells acquire spontaneous recombination- and SOS response-independent β-lactam resistance mutations in ampD . This chromosomal system is useful for studying mutation mechanisms that promote antibiotic resistance.