Background: Mycoplasma pneumonia (M. pneumoniae) is an important causative agent of childhood infection with variable clinical presentations. The objective of the study was to evaluate the laboratory investigation and antimicrobial therapy of M. pneumoniae infection in children admitted to paediatric ward. Materials & Methods: A total of 111 children, of which 59 (53.2%) boys and 52 (46.8%) girls, with median age of 2 years (inter quartile range 1-6 years) with suspected M. pneumoniae infection were tested for IgM by enzyme-linked immunosorbent assay (ELISA). The children were classified as seropositive and seronegative. Results: Of the 111 children, 45 (40.5%) had serological evidence of M. pneumoniae infection and the remaining 66 (59.5%) were seronegative. There was significant association (p