The distress call is characterized by loud, explosive calls emitted in response to disturbance or potential predators. Herein we describe the distress call of a female of Hypsiboas leucocheilus from Aripuanã, north of Mato Grosso state, Brazil. The call is emitted with the mouth open and consists of a single, not pulsed, harmonic note. This call is similar to other distress calls known for Brazilian hylids.