This work investigates the use of supported liquid membranes (SLM) in the pertraction of fructose from a mixture of sugars contained in a fermentation broth. Membranes consisted in a porous polypropylene support impregnated with different kinds of carriers using 2-nitrophenyl octyl ether as solvent. Transport through flat sheet and hollow-fiber membranes was studied as a function of carrier and feed concentration. The results show that a boronic acid derivative yields the highest fructose selectivity and the hollow fiber supported liquid membrane (HFSLM) is more stable than the flat sheet system, also yielding higher fructose selectivities using lower carrier concentration. The HFSLM using a boronic acid as carrier was able to remove fructose selectively from a fermentation broth. Simulations of fructose removal from a fermentation broth were carried out using the experimental results obtained in this work. The results show that fructose removal from the fermentation broth can reduce microorganism inhibition and increase the system performance, although, further improvement in membrane stability and fluxes are still necessary.