Rhodamines NN: A Novel Class of Caged Fluorescent Dyes
Journal article published in 2010 by
Vladimir N. Belov 
,
Christian A. Wurm,
Vadim P. Boyarskiy,
Stefan Jakobs ,
Stefan W. Hell
,
Christian A. Wurm,
Vadim P. Boyarskiy,
Stefan Jakobs ,
Stefan W. Hell
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Abstract
(Figure Presented). A bright future: The reaction of diazomethane with acid chlorides obtained from N,N,N′,N′-tetraalkylrhodamines affords 2-diazo-2,3-dihydro-1Hindenespiro[1,9′]-9H-xanthen-3-ones-a novel class of caged rhodamines. These dyes allow new imaging procedures based on the step-wise activation and detection of several fluorescent markers (see picture).