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American Chemical Society, Accounts of Chemical Research, 7(38), p. 542-548, 2005

DOI: 10.1021/ar040138c

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Surfaces and Orientations: Much to FRET about?

Journal article published in 2005 by Ivan Rasnik, Sean A. McKinney, Taekjip Ha ORCID
This paper is available in a repository.
This paper is available in a repository.

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Abstract

Single molecule FRET (fluorescence resonance energy transfer) is a powerful technique for detecting real-time conformational changes and molecular interactions during biological reactions. In this Account, we examine different techniques of extending observation times via immobilization and illustrate how useful biological information can be obtained from single molecule FRET time trajectories with or without absolute distance information.