Published in
Springer Verlag, Transgenic Research, 4(4), p. 288-290
DOI: 10.1007/bf01969123
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pBINPLUS: an improved plant transformation vector based on pBIN19.
Journal article published in 1970 by
Fred A. van Engelen,
Jos W. Molthoff,
Anthony J. Conner,
Jan-Peter Nap,
Andy Pereira 
,
Willem J. Stiekema
,
Willem J. Stiekema
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Abstract
We describe the construction of a new plant transformation vector, pBINPLUS, based on the popular pBIN19 vector. Improvements over pBIN19 include location of the selectable marker gene at the left T-DNA border, a higher copy number in E. coli, and two rare restriction sites around the multiple cloning site for easier cloning and analysis of T-DNA insertions in plant genomes.