In this paper we report the preparation and characterization of ceramic-polymer composites doped with gold nanoparticles. Properties and applications in medicine and dentistry of colloidal gold nanoparticles depend upon their size and shape. The influence of metallic nanoparticles on the in vitro behavior was investigated by pH analyses of simulated body fluid and artificial saliva. The nanocomposites were characterized with the use of X-ray Diffaction (XRD) and Fourier Transformed Infrared Spectroscopy (FT-IR) methods. The results of in vitro tests confirmed that it is possible to produce hydroxyapatite/polymer (HA/polymer) composites doped with gold nanoparticles (AuNPs) for medical applications. Investigations proved that presence of gold nanoparticles in composites had influence on in vitro behaviour of HA/Polymer/AuNPs composites in artificial physiological fluids. Research results indicate that obtained composites demonstrate bioactivity features and could become innovative materials for biomedical and dentist applications.