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Published in

Microbiology Society, Journal of General Virology, 7(94), p. 1680-1689, 2013

DOI: 10.1099/vir.0.053850-0

Microbiology Society, Journal of General Virology, 1(95), p. 244-244, 2014

DOI: 10.1099/vir.0.060921-0

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Knockdown of piRNA pathway proteins results in enhanced Semliki Forest virus production in mosquito cells

Journal article published in 2013 by Esther Schnettler, Claire L. Donald, Stacey Human ORCID, Mick Watson, Ricky W. C. Siu, Melanie McFarlane, John K. Fazakerley ORCID, Alain Kohl, Rennos Fragkoudis
This paper is available in a repository.
This paper is available in a repository.

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Abstract

The exogenous siRNA pathway is important in restricting arbovirus infection in mosquitoes. Less is known about the role of the PIWI-interacting RNA pathway, or piRNA pathway, in antiviral responses. Viral piRNA-like molecules have recently been described following infection of mosquitoes and derived cell lines with several arboviruses. The piRNA pathway has thus been suggested to function as an additional small RNA-mediated antiviral response to the known infection-induced siRNA response. Here we show that piRNA-like molecules are produced following infection with the naturally mosquito-borne Semliki Forest virus in mosquito cell lines. We show that knockdown of piRNA pathway proteins enhances the replication of this arbovirus and defines the contribution of piRNA pathway effectors, thus characterizing the antiviral properties of the piRNA pathway. In conclusion, arbovirus infection can trigger the piRNA pathway in mosquito cells, and knockdown of piRNA proteins enhances virus production.