Crop monitoring is important in precision agriculture. Estimating above-ground biomass helps to monitor crop vitality and to predict yield. In this study, we estimated fresh and dry biomass on a summer barley test site with 18 cultivars and two nitrogen (N)-treatments using the plant height (PH) from crop surface models (CSMs). The super-high resolution, multi-temporal (1 cm/pixel) CSMs were derived from red, green, blue (RGB) images captured from a small unmanned aerial vehicle (UAV). Comparison with PH reference measurements yielded an R 2 of 0.92. The test site with different cultivars and treatments was monitored during "Biologische Bundesanstalt, Bundessortenamt und CHemische Industrie" (BBCH) Stages 24–89. A high correlation was found between PH from CSMs and fresh biomass (R 2 = 0.81) and dry biomass (R 2 = 0.82). Five models for above-ground fresh and dry biomass estimation were tested by cross-validation. Modelling biomass between different N-treatments for fresh biomass produced the best results (R 2 = 0.71). The main limitation was the influence of lodging cultivars in the later growth stages, producing irregular plant heights. The method has potential for future application by non-professionals, i.e., farmers.