Published in
Nature Research, Nature Methods, 5(12), p. 415-418, 2015
DOI: 10.1038/nmeth.3339
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Quantifying cellular capacity identifies gene expression designs with reduced burden
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Abstract
Heterologous gene expression can be a significant burden for cells. Here we describe an in vivo monitor that tracks changes in the capacity of Escherichia coli in real time and can be used to assay the burden imposed by synthetic constructs and their parts. We identify construct designs with reduced burden that predictably outperformed less efficient designs, despite having equivalent output.