PSAAL (Physiological Signals Acquisition and Analysis in LabVIEW language) is a group of seven programs, written in LabVIEW 3.0 language and specialized for acquisition, analysis, reduction, and display of peripheral and central physiological data. The main characteristics of the programs are the wide range of use for most short-duration psychophysiological paradigms and the easy-to-use graphic interface. In addition, two noteworthy features of LabVIEW are included: the portability of the programs on Macintosh, PowerMac, IBM, HP-UX, and SUN computers, and the capability of handling acquisition on many National Instruments A/D boards, including the low-cost ones. PSAAL performs acquisition and simple reduction of peripheral physiological responses with 12- or 16-bit resolution according to the A/D board used. Analysis includes conversion of electrocardiogram into heart rate, the elaboration of the main parameters of skin conductance responses, electromyogram, startle reflex, and averaging of evoked potentials or other event-related physiological responses, under different experimental conditions. The programs allow the user to perform the organization of the data in a spreadsheet ready for most statistical packages and without the use of other spreadsheet applications.