The effect of devegetation on the microbiological and biochemical quality of a soil in a semiarid climate was studied 15 years later. In this climate, devegetated soils do not generate spontaneous vegetation and this leads to diminished carbon fractions. Microbial activity as determined by parameters of general microbial activity, such as basal respiration and ATP, confirms the negative effect of devegetation on the microbiological quality of the soil. This negative effect also is reflected in microbial biomass C, which acts as an indicator of microbial population size. Specific parameters, such as oxidoreductase enzymatic activities (dehydrogenase), or hydrolases of the N cycle (protease that hydrolyses N-α benzoyl-L-argininamide), also point to the negative effect of devegetation on soil biochemical quality. All this was in evidence despite the fact that 15 years had passed since devegetation.