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BioMed Central, BMC Cancer, 1(12), p. 428

DOI: 10.1186/1471-2407-12-428

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Pleural fluid cell-free DNA integrity index to identify cytologically negative malignant pleural effusions including mesotheliomas

Journal article published in 2012 by Krishna B. Sriram, Vandana Relan, Belinda E. Clarke, Edwina E. Duhig, Morgan N. Windsor, Kevin S. Matar, Rishendran Naidoo, Linda Passmore, Elizabeth McCaul, Deborah Courtney, Ian A. Yang ORCID, Rayleen V. Bowman, Kwun M. Fong
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Abstract Background The diagnosis of malignant pleural effusions (MPE) is often clinically challenging, especially if the cytology is negative for malignancy. DNA integrity index has been reported to be a marker of malignancy. The aim of this study was to evaluate the utility of pleural fluid DNA integrity index in the diagnosis of MPE. Methods We studied 75 pleural fluid and matched serum samples from consecutive subjects. Pleural fluid and serum ALU DNA repeats [115bp, 247bp and 247bp/115bp ratio (DNA integrity index)] were assessed by real-time quantitative PCR. Pleural fluid and serum mesothelin levels were quantified using ELISA. Results Based on clinico-pathological evaluation, 52 subjects had MPE (including 16 mesotheliomas) and 23 had benign effusions. Pleural fluid DNA integrity index was higher in MPE compared with benign effusions (1.2 vs. 0.8; p