AbstractActivity patterns of the sand viper, Vipera ammodytes from the central Balkans (Serbia, Montenegro and Former Yugoslav Republic of Macedonia) were analysed. The total sample (n = 118) was divided into adult males and females, and depending on the part of the day, habitat type, exposition, and behaviour in two separate seasons (spring and summer). Simple correspondence analysis showed that in spring, males and females were associated with different expositions, while in summer, they displayed different behaviours. Multivariate correspondence analysis showed that in spring males were found more often while basking during the midday in the open forests at south-western exposition. In summer, females were found more often while basking or hiding in the morning in rocky terrains at eastern and southern expositions. The results point out that seasonal variation and intergender differences in behaviour and microhabitat use may occur in sand vipers, as was previously observed in other viper species.