The Old Crow basin, northern Yukon, provides a key record of environmental change in northwestern North America from the late-Middle Pleistocene through the Early Holocene. Site chronologies are based on the presence of the Old Crow tephra (OCt: 124 ` 10 ka) at three sites, and provide a stratigraphic framework for observations. Ecological affinities of fossil insects indicate that Pleistocene environments were dominated by dry tundra and steppe-tundra. Forest insect species are relatively rare even during the last interglaciation (MIS 5e), and it is only by considering the total insect assemblages that inter- glacial beds can be recognized. Last interglacial insects from these sites indicate a relatively wet and warm tundra environment with evidence for sparse forest vegetation. Rare taxa with strong affinities to warmer temperatures are present and could suggest a warmer than modern climate. Early Holocene deposits yield a greater abundance of forest insects relative to MIS 5 or 7 interglacial deposits. Fossil insect assemblages reflect the position of the sites above the Arctic Circle, and in contrast to central Yukon sites, steppe insects are less common during cold stages and forest insects are less common during warm stages. These data suggest overall that the contrast between cold and warm stages was less pronounced than in other regions of Yukon and Alaska, and may indicate influence of persistent large regional lakes during the Pleistocene.