Abstract We report the CH4 emission flux from the city of Indianapolis, IN, the site of the Indianapolis Flux Experiment (INFLUX) project for developing, assessing, and improving top-down and bottom-up approaches for quantifying urban greenhouse gas emissions. Using an aircraft-based mass balance approach, we find that the average CH4 emission rate from five flight experiments in 2011 is 135 ± 58 (1σ) moles s-1 (7800 ± 3300 kg hr-1). The effective per capita CH4 emission rate for Indianapolis is 77 kg CH4 person-1 yr-1, a figure that is less than the national anthropogenic CH4 emission (∼91 kg CH4 person-1 yr-1) but considerably larger than the global figure (∼48 kg CH4 person-1 yr-1). We consistently observed elevated CH4 concentrations at specific coordinates along our flight transects downwind of the city. Inflight investigations as well as back trajectories using measured wind directions showed that the elevated concentrations originated from the southwest side of the city where a landfill and a natural gas transmission regulating station (TRS) are located. Street level mobile measurements downwind of the landfill and the TRS supported the results of aircraft-based data, and were used to quantify the relative contributions from the two sources. We find that the CH4 emission from the TRS was negligible relative to the landfill, which was responsible for 33 ± 10% of the citywide emission flux. A regression of propane versus methane from aircraft flask samples suggests that the remaining citywide CH4 emissions (∼67%) derive from the natural gas distribution system. We discuss the combination of surface mobile observations and aircraft city-wide flux measurements to determine the total flux and apportionment to important sources.