Nanostructured alpha-nickel hydroxide (α-Ni(OH)2) immobilized on a Fluorine-doped Tin Oxide (FTO) surface was explored for the construction of hydrogen peroxide amperometric Flow Injection Analysis (FIA) sensors. Their notable electrocatalytic activity and heterogeneous electron-transfer rate were confirmed by the appearance of a broad and intense peak associated with the oxidation of hydrogen peroxide and the enhancement of sensibility in hydrodynamic conditions. The α-Ni(OH)2 electrodes exhibited a broad dynamic range (5×10−6 to 1×10−3 mol L−1), low detection limit (2×10−7 mol L−1), good repeatability (RSD=1.29 % for 20 successive analyses), and a sensitivity greater than 500 µA mmol−1 L−1 cm−2.