Electrocatalysis at nanoparticles modified electrode has recently received huge attention because of the development of new sensors and energy conversion systems. The employment of two metals, with different catalytic properties are sometimes superior from the point of view reaction efficiency and overpotential decrease. Here, we demonstrate how electrocatalytic behavior of bimetallic noble metal nanostructures prepared from two types of nanoparticles are superior as compared with prepared from single type nanoparticles on the example of glucose oxidation. The bimetallic nanoparticulate films were prepared on indium tin dioxide substrates by droplet deposition or layer-by-layer method. The bi-metallic nanoparticulate films were characterized by scanning electron microscopy, X-ray powder diffraction and X-ray photoelectron spectroscopy. Electrocatalytic oxidation of glucose was studied by cyclic voltammetry and chronoamperometry. The efficiency of this reaction is much higher in the case of bimetallic film as compared with films prepared from single type of nanoparticles. Some impact of the charge of stabilizing groups of nanoparticles and the method of preparation on the efficiency of glucose oxidation is seen.