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Optica, Optics Express, 23(22), p. 29191, 2014

DOI: 10.1364/oe.22.029191

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Stochastic 3D optical mapping by holographic localization of Brownian scatterers

This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We present a wide-field microscopy technique for the 3D mapping of optical intensity distributions using Brownian gold nanopar-ticles as local probes, which are localized by off-axis holography. Fast computation methods allow us to localize hundreds of particles per minute with accuracies as good as 3 × 3 × 10nm3 for immobilized particles. Factors limiting this accuracy are discussed and the possibilities of the technique are illustrated through the 3D optical mapping of an evanescent and a propagative wave. Our results pave the way for a new stochastic imaging technique, well adapted to subwavelength optical characterization in water-based systems.