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Nature Research, Nature Methods, 9(3), p. 721-723, 2006

DOI: 10.1038/nmeth922

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Nanoscale resolution in GFP-based microscopy

Journal article published in 2006 by Katrin I. Willig, Robert R. Kellner, Rebecca Medda, Birka Hein, Stefan Jakobs ORCID, Stefan W. Hell
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This paper is available in a repository.

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Abstract

We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The approximately 70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers.