The essential oil from the leaves of Annona vepretorun was obtained by hydrodistillation using a Clevenger-type apparatus and analyzed by GC-MS and GC-FID. Eighteen compounds representing 98.1% of the crude essential oil were identified. The major compounds identified were bicyclogermacrene (43.7%), spathulenol (11.4%), α-felandrene (10.0%), α-pinene (7.1%), (E)-β-ocimene (6.8%), germacrene D (5.8%), and p-cymene (4.2%). The trypanocidal activity against Trypanosoma cruzi epimastigote forms, as well as, the antimicrobial and antioxidant proprieties was investigated. The essential oil showed a potent trypanocidal activity with IC₅₀ value of 31.9±1.3μg.mL^-1. For antimicrobial activity, the best result was observed against Candida tropicalis with a MIC value of 100μg.mL^-1. For antioxidant capacity the essential oil showed weak activity.