This study was conducted to estimate the concentration of essential oil and some heavy metals (Cd, Pb, Ni, Mn, Zn & Cu) in thyme plant (Thymus vulgaris) cultivated in the southern (Maan, Al-Shouback & Al-Tafeileh), middle (Amman) and northern (Jeresh, Ajlune & Irbid) regions of Jordan. The results showed a wide variation of essential oil values yielded from thyme plant. The mean value of essential oil was 4.63% at the north environment, while it was decreased to 2.77 and 2.70% in the south and middle regions, respectively. The results showed differences in the metal concentrations of all samples. The highest concentrations (in mg kg -1) of Ni (22.21), Cu (7.97) and Zn (48.73) were recorded at the north environment of Jordan but they were within permissible limit for medicinal plants. Cadmium (Cd) in all specimens of T. vulgaris collected from the completely cultivated areas of Jordan was non-detectable (<1.20 mg kg -1). The average concentrations of Pb were 33.31, 22.38, 29.46 mg kg -1 , at the north, middle and south cultivated areas, respectively which were higher than permissible limits. In conclusion the essential oil and heavy metals contents in T. vulgaris are mainly affected by variable natural climatic conditions.