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Humana Press, Methods in Molecular Biology, p. 123-135, 2015
DOI: 10.1007/978-1-4939-2620-6_10
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Q-Bank Phytoplasma: A DNA Barcoding Tool for Phytoplasma Identification
,
Assunta Bertaccini,
Mogens Nicolaisen
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Abstract
DNA barcoding is an identification method based on comparison of a short DNA sequence with known sequences from a database. A DNA barcoding tool has been developed for phytoplasma identification. This phytoplasma DNA barcoding protocol based on the tuf gene has been shown to identify phytoplasmas belonging to the following groups: 16SrI, 16SrII, 16SrIII, 16SrIV, 16SrV, 16SrVI, 16SrVII, 16SrIX, 16SrX, 16SrXI, 16SrXII, 16SrXIV, 16SrXX, and 16SrXXI.