The present review is an update of the previous one published in Proteomics 2015 Reviews special issue (1) covering the 2014-July 2015 period. It has been written on the bases of the publications that appeared in Proteomics journal during that period and the most relevant ones that have been published in other high-impact journals. Methodological advances and the contribution of the field to the knowledge of plant biology processes and its translation to agroforestry and environmental sectors will be discussed. This review has been organized in four blocks, with a starting general introduction (literature survey) followed by sections focusing on the methodology (in vitro, in vivo, wet and dry), proteomics integration with other approaches (systems biology and proteogenomics), biological information, and knowledge (cell communication, receptors and signaling), ending with a brief mention of some other biological and translational topics to which proteomics has made some contribution. This article is protected by copyright. All rights reserved