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Public Library of Science, PLoS ONE, 9(10), p. e0137784, 2015

DOI: 10.1371/journal.pone.0137784

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A Metagenomic Investigation of the Duodenal Microbiota Reveals Links with Obesity

Journal article published in 2015 by Emmanouil Angelakis, Fabrice Armougom, Frédéric Carrière ORCID, Dipankar Bachar, René Laugier, Jean-Christophe Lagier, Catherine Robert, Caroline Michelle, Bernard Henrissat, Didier Raoult
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Background Few studies have tested the small intestine microbiota in humans, where most nutrient digestion and absorption occur. Here, our objective was to examine the duodenal microbiota between obese and normal volunteers using metagenomic techniques. Methodology/Principal Findings We tested duodenal samples from five obese and five normal volunteers using 16S rDNA V6 pyrosequencing and Illumina MiSeq deep sequencing. The predominant phyla of the duodenal microbiota were Firmicutes and Actinobacteria, whereas Bacteroidetes were absent. Obese individuals had a significant increase in anaerobic genera (p