The paper presents results on the occurrence of three- (or more) needle dwarf shoots on two-needle pine species in southern and central Europe. The anomalous dwarf shoots were found on single individuals of Pinus halepensis and P. nigra from Greece, and on P. sylvestris from Spain. The significantly high level of frequency of individuals with at least one anomalous three-needle dwarf shoot were confirmed for P. mugo subsp. uncinata from western and for P. mugo subsp. mugo from central Europe. The frequency of individuals with anomalous dwarf shoots on the latter species in the Balkan populations is statistically significantly lower than in the Alpine, Sudethian and Carpathian ones. The highest frequencies of individuals with three-needle dwarf shoots on P. mugo subsp. mugo were found in the northernmost populations, in the Sudethian and west Carpathian ones, and in the locations near the upper limit of the species.