The use of drug-loaded nanoparticles and microparticles has been increasing, especially for cosmetic and drug delivery purposes. In this work, a new microparticle formulation was developed for use in the healing process of skin burns in a composition of Aloe vera/ vitamin E /chitosan. In order to observe the morphological properties, Raman and Atomic Force Microscopy evaluation were performed.The biodistribution studies were analysed by using a nuclear methodology, labeling the microparticles with Techentium-99m and in vivo test was procedure to analysed the cicatrization process. The results of AFM analysis show the formation and the adherence property of the micropaticles. Raman analyses show the distribution of each component in the microparticle. The nuclear method used shows that the biodistribution of the microparticles remained in the skin. The in vivo cicatrization test showed that the poloxamer gel containing the microparticles make a better cicatrization in relation the other formulations tested.