This work aimed to evaluate nutritional condition of Italia grapevine in the region centered in São Miguel Arcanjo, SP, Brazil. It was used the Diagnosis and Recommendation Integrated System (DRIS). Twenty bearing vineyards in that region using similar cultural practices were nutritionally surveyed. Leaf blade and petiole samples were taken at three developing phases of grapevine plants: at bloom, in fruits between pea and have-berry and at the beginning of fruit maturation. Leaf blade and petiole samples were analyzed for macronutrients. The reference population used in the calculation of DRIS norms was that of vineyards with average yield between 20.7 to 36.3t/ha. NBI (Nutritional Balance Index) and the sequence of nutrient limitation were individually calculated for each vineyard at three sample phases and for leaf blade and petiole. In this evaluation permitted to conclude that DRIS was a good method for evaluation of nutritional condition of grapevine cv. Italia. Eleven vineyards were considered nutritionally balanced, with average yield between 20.7 to 36.3t/ha. Correlations between NBI and yield indicated that leaf blade sampled at bloom was the better choice. The DRIS allowed the determination of vineyards that usually presented a deficiency of phosphorus, potassium and magnesium.