Tetraploa aristata Berkeley & Broome represents the anamorphic stage of a pleomorphic fungus with holomorph Tetraplosphaeria tetraploa (Scheuer) Kaz. Tanaka & K. Hiray (Lophiostoma tetraploa (Scheuer) Aptroot & K.D. Hyde). Until now this taxon has not been reported in Poland, where it is probably on the fringe of its distribution. Conidia of Tetraploa aristata were found during palynological analyses of modern surface samples and later on the decaying leaves of Phleum phleoides from xeric grasslands in Krakow and its vicinity. Since Tetraploa aristata was discovered during palynological analysis, this method might be considered to be a useful tool for investigations of contemporary microfungal mycobiota.