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Lifescience Global, International Journal of Statistics in Medical Research, 2012

DOI: 10.6000/1929-6029.2012.01.02.10

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Analysis of RT-qPCR Data

Journal article published in 2012 by Kohl ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We give a brief overview of the necessary steps in the analysis of real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) data. We cover determination of amplification efficiency, background correction, normalization, quality control, and statistical analysis.