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Springer, Plant Cell Reports, 12(25), p. 1325-1335, 2006

DOI: 10.1007/s00299-006-0188-4

Humana Press, Methods in Molecular Biology, p. 151-161, 2012

DOI: 10.1007/978-1-61779-558-9_13

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Transformation of barley (Hordeum vulgare L.) by Agrobacterium tumefaciens infection of in vitro cultured ovules

Journal article published in 2006 by Inger Bæksted Holme, Henrik Brinch-Pedersen ORCID, Mette Lange, Preben Bach Holm
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We report on a novel transformation procedure for barley by Agrobacterium infection of in vitro cultured ovules. Ovules of the cultivar Golden Promise were isolated a few hours after pollination and infected with the Agrobacterium tumefaciens strain AGL0 carrying the binary vector pVec8-GFP. The vector harboured a hygromycin resistance gene and the green fluorescence protein (GFP) gene. GFP-expressing embryos were isolated from the ovules, regenerated to plants and investigated by Southern blot analysis. Transformation frequencies amounted to 3.1% with hygromycin selection and 0.8% without selection. Mendelian inheritance and stable expression of the GFP gene was confirmed in 18 independent lines over two generations. We conclude that the described technique allows for the rapid and direct generation of high quality transgenic plants.