Background: Although pharmacists have been granted prescribing privileges in Alberta since 2007 and most other Canadian provinces have granted or put forward similar legislation since then, there is a lack of Canadian data exploring pharmacists' understanding and adoption of prescribing practice. Therefore, our goal is to investigate pharmacists' perceptions of prescribing, the extent to which prescribing has been incorporated into pharmacists' practices and the factors that have influenced its uptake. Methods (Study Design): We are conducting a mixed-methods evaluation of pharmacist prescribing in Alberta and Ontario consisting of 3 consecutive stages: 1) semi-structured interviews with a small cohort of pharmacists in Alberta and Ontario; 2) development of a survey guided by responses identified in Stage 1; and 3) a mixed-methods survey of a large random sample of pharmacists in Alberta. Conclusion: When complete, this study will inform researchers, policy-makers and educators about Ontario pharmacists' attitudes towards prescribing and the initial uptake of prescribing practice in Alberta. This will allow for a greater understanding of pharmacists' perceptions of prescribing; the extent to which prescribing has been incorporated into pharmacy practice; and the factors facilitating its uptake.