Alcohols (ethanol and methanol) are being widely considered as alternative fuels for automotive applications. At the same time, homogeneous charge compression ignition (HCCI) engine has attracted global attention due to its potential of providing high engine efficiency and ultralow exhaust emissions. Environmental legislation is becoming increasingly stringent, sharply focusing on particulate matter (PM) emissions. Recent emission norms consider limiting PM number concentrations in addition to PM mass. Therefore, present study is conducted to experimentally investigate the effects of engine operating parameters on the PM size–number distribution in a HCCI engine fueled with gasoline, ethanol, and methanol. The experiments were conducted on a modified four-cylinder diesel engine, with one cylinder modified to operate in HCCI mode. Port fuel injection was used for preparing homogeneous charge in the HCCI cylinder. Intake air preheating was used to enable auto-ignition of fuel–air mixture. Engine exhaust particle sizer (EEPS) was used for measuring size–number distribution of soot particles emitted by the HCCI engine cylinder under varying engine operating conditions. Experiments were conducted at 1200 and 2400 rpm by varying intake air temperature and air–fuel ratio for gasoline, ethanol, and methanol. In this paper, the effect of engine operating parameters on PM size–number distribution, count mean diameter (CMD), and total PM numbers is investigated. The experimental data show that the PM number emissions from gasoline, ethanol, and methanol in HCCI cannot be neglected and particle numbers increase for relatively richer mixtures and higher intake air temperatures.