Dissemin is shutting down on January 1st, 2025

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Nature Research, Nature Methods, 11(5), p. 943-945, 2008

DOI: 10.1038/nmeth.1257

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Fluorescence nanoscopy by ground-state depletion and single-molecule return

This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Data provided by SHERPA/RoMEO

Abstract

We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine- and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach.