Here, we report the tuning of active reaction center of the CoSe₂ nanoparticles by nitrogen doped carbon nanohorns (NCNH). CoSe₂ nanoparticles supported on NCNH (CoSe₂/NCNH) shows improved oxygen reduction reaction (ORR) activity compared to the same supported on carbon nanohorns and Vulcan carbon. An improved positive shift in onset potential (50 mV) and half-wave potential (150 mV) of CoSe₂/NCNH compared to other catalysts indicates the intrinsic activity as well as active reaction center of CoSe₂ modulated by the presence of NCNH. CoSe₂/NCNH reduces molecular oxygen to hydroxide through a four-electron transfer pathway with improved electrochemical stability in alkaline condition. Air breathing direct methanol micro laminar flow fuel cells (DM-µLFFC) performance of CoSe₂/NCNH as cathode catalyst shows a maximum power density and current density respectively of 10.05 mW cm^-2 and 124.20 mA cm^-2 with 5M methanol as fuel.