Elsevier, Cancer Letters, 2(343), p. 275-285, 2014
DOI: 10.1016/j.canlet.2013.09.029
Full text: Download
The discovery of novel markers for Breast Cancer (BC) has been recently relied on antibody combinatorial libraries and selection through Phage Display. We constructed a recombinant Fab library, and after selections against BC tissues, the FabC4 clone was thoroughly investigated by immunohistochemistry in 232 patients with long-term follow-up. The FabC4 ligand was determined by mass spectrometry. The FabC4 expression was associated with younger age, lack of progesterone receptor, higher histological grades and non-luminal subtypes, and it also identified a subset of good prognostic triple-negative BCs, possibly targeting a conformational epitope of Cytokeratin-10 (CK10). This new CK10-epitope specific antibody may open new possibilities in diagnostic and therapeutic strategies.