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Wiley, Insect Molecular Biology, 6(18), p. 747-757, 2009

DOI: 10.1111/j.1365-2583.2009.00917.x

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Molecular analysis of a mutantAnticarsia gemmatalis multiple nucleopolyhedrovirus(AgMNPV) shows an interruption of an inhibitor of apoptosis gene (iap-3) by a new class-IIpiggyBac-related insect transposon

Journal article published in 2009 by M. P. Carpes, J. F. Nunes, T. L. Sampaio, M. E. B. Castro, P. M. A. Zanotto, B. M. Ribeiro ORCID
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

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Abstract

A new piggyBac-related transposable element (TE) was found in the genome of a mutant Anticarsia gemmatalis multiple nucleopolyhedrovirus interrupting an inhibitor of apoptosis gene. This mutant virus induces apoptosis upon infection of an Anticarsia gemmatalis cell line, but not in a Trichoplusia ni cell line. The sequence of the new TE (which was named IDT for iap disruptor transposon) has 2531 bp with two DNA sequences flanking a putative Transposase (Tpase) ORF of 1719 bp coding for a protein with 572 amino acids. These structural features are similar to the piggyBac TE, also reported for the first time in the genome of a baculovirus. We have also isolated variants of this new TE from different lepidopteran insect cells and compared their Tpase sequences.