Published in

BioMed Central, Lipids in Health and Disease, 1(13), 2014

DOI: 10.1186/1476-511x-13-43

Links

Tools

Export citation

Search in Google Scholar

The effects of obesity, diabetes and metabolic syndrome on the hydrolytic enzymes of the endocannabinoid system in animal and human adipocytes

Journal article published in 2014 by Jemma C. Cable, Garry D. Tan, Stephen Ph H. Alexander ORCID, Saoirse Elizabeth O'Sullivan, Saoirse E. O. Sullivan
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

Full text: Download

Green circle
Preprint: archiving allowed
Upload
Green circle
Postprint: archiving allowed
Upload
Green circle
Published version: archiving allowed
Upload
Policy details
Data provided by SHERPA/RoMEO

Abstract

Abstract Background Circulating endocannabinoid levels are increased in obesity and diabetes. We have shown that fatty acid amide hydrolase (FAAH, an endocannabinoid hydrolysing enzyme) in subcutaneous adipose tissue positively correlates with BMI in healthy volunteers. The aim of the present study was to investigate whether the hydrolytic enzymes of the endocannabinoid system are affected by diabetes or metabolic syndrome in obesity. Methods Using radiolabelled substrates, FAAH and monoacylglycerol lipase (MGL) activities were assessed in adipocytes from various adipose depots in Zucker rats (n = 22, subcutaneous abdominal, visceral and epididymal) and bariatric patients (n = 28, subcutaneous abdominal and omental). Results FAAH activity was significantly increased in adipocytes of obese (Zucker Fatty) compared to Zucker lean rats (P