Published in

Oxford University Press, FEMS Microbiology Letters, 1(176), p. 51-56, 1999

DOI: 10.1111/j.1574-6968.1999.tb13641.x

Oxford University Press (OUP), FEMS Microbiology Letters, 1(176), p. 51-56

DOI: 10.1016/s0378-1097(99)00218-9

Links

Tools

Export citation

Search in Google Scholar

Green fluorescent protein (GFP) as gene expression reporter and vital marker for studying development and microbe-plant interaction in the tobacco pathogenPhytophthora parasiticavar.nicotianae

Journal article published in 1999 by Arnaud Bottin, Lydie Larche, François Villalba, Elodie Gaulin ORCID, Marie-Thã©rã¨se Esquerré-Tugayé, Martina Rickauer
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

Full text: Unavailable

Green circle
Preprint: archiving allowed
Upload
Orange circle
Postprint: archiving restricted
Upload
Red circle
Published version: archiving forbidden
Policy details
Data provided by SHERPA/RoMEO

Abstract

PEG-mediated transformation of protoplasts in the presence of lipofectin was achieved in Phytophthora parasitica var. nicotianae, an oomycete pathogen of tobacco. Using oomycete promoter and terminator sequences, a plant-adapted green fluorescent protein (GFP) was introduced into the microorganism. The data show for the first time that this eukaryotic gene reporter can be used in an oomycete, both as a quantitative reporter of gene induction and as a vital marker allowing the study of development of Phytophthora in vitro and in the host plant.