In this study, microalgae were cultivated in the form of suspension cultures using a new structurally organized immobilization technique called “Green Bioprinting”. This technique allows the co-cultivation of different microorganisms in close vicinity to, but without direct contact with microalgae. The goal is to improve the oxygen supply of different cell types by photosynthetic oxygen evolution. However, more information on the optimum culture conditions for different microalgae is necessary. Therefore, Chlamydomonas reinhardtii 11.32b and Chlorella sorokiniana UTEX1230 were suspended in culture medium or embedded in hydrogels by the 3 D-bioprinting process followed by cultivation under different temperatures (26°C, 30°C or 37°C) and modes of illumination (continuous illumination or a 14/10 hours light/dark cycle). The viability was monitored by either flow cytometry (suspension cultures) analysis of DiBAC4(3)-stained cells or fluorescence image analysis (hydrogel-embedded cultures). Suspended microalgae subjected to continuous illumination exhibited an increased number of membrane-depolarized cells compared to those cultivated at a 14/10 hours light/dark cycle. Hydrogel-immobilization resulted in a facilitated viability and stable growth rates between 0.4 d−1 and 0.7 d−1 for both microalgae strains. Concluding, the 3 D-bioprinting immobilization represents a technique to cultivate microalgae at a high viability and growth rate even under non-optimal temperature conditions.This article is protected by copyright. All rights reserved