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Instituto Oswaldo Cruz, Ministério da Saúde, Memórias do Instituto Oswaldo Cruz., 5(108), p. 631-636, 2013

DOI: 10.1590/0074-0276108052013015

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Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)

Journal article published in 2013 by Carla Vanessa de Paula Lima, Lima,Carla Vanessa de Paula, Fragoso Stênio Perdigão, Ligia Cristina Kalb Souza, Souza,Ligia Cristina Kalb, Rosana Elisa Gonçalves Goncalves Pinho, Rosana Elisa Gonçalves Gonçalves Pinho, Pinho,Rosana Elisa Gonçalves Gonçalves, Carla Vanessa de Paula Lima, Stenio Perdigao Fragoso ORCID, Soares,Maurilio José, Maurilio José Soares ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Heteroxenic and monoxenic trypanosomatids were screened for the presence of actin using a mouse polyclonal antibody produced against the entire sequence of the Trypanosoma cruzi actin gene, encoding a 41.9 kDa protein. Western blot analysis showed that this antibody reacted with a polypeptide of approximately 42 kDa in the whole-cell lysates of parasites targeting mammals (T. cruzi, Trypanosoma brucei and Leishmania major ), insects ( Angomonas deanei , Crithidia fasciculata , Herpetomonas samuelpessoai and Strigomonas culicis ) and plants ( Phytomonas serpens ). A single polypeptide of approximately 42 kDa was detected in the whole-cell lysates of T. cruzi cultured epimastigotes, metacyclic trypomastigotes and amastigotes at similar protein expression levels. Confocal microscopy showed that actin was expressed throughout the cytoplasm of all the tested trypanosomatids. These data demonstrate that actin expression is widespread in trypanosomatids.