Electrochemical characteristics of rutin, as an important biological molecule, have been studied at a glassy carbon electrode using cyclic voltammetry and chronocoulometry methods. The redox response of rutin solution at an inactivated glassy carbon electrode (IGCE) was investigated and E r C i mechanism was deduced for rutin oxidation by voltammetric data at various scan rates and in different rutin concentrations. The results show that the subsequent chemical reactions of the E r C i mechanism for rutin oxidation at an IGCE are dimerization and intramolecular reactions. Also, it has been shown that the oxidation of rutin at an activated glassy carbon electrode (AGCE) leads to the formation of a deposited layer which shows one pair of peaks with surface confined characteristics. The surface excess of bonded rutin, Γ R , and diffusion coefficient, D, for free rutin were determined as 5.8×10 -11 mol cm -2 1.9×10 -5 cm 2 s -1 respectively from chronocoulometry experiments. The heterogeneous charge transfer rate constant, k s , and the transfer coefficient, α, for electron transfer between bonded rutin and the AGCE were calculated as 98.0 ± 2.0 s -1 and 0.48 respectively. The results indicate that the k s of bonded rutin at an AGCE is almost twice larger than reported for adsorbed rutin at a multi-wall carbon nanotubes (MWCNT) glassy carbon electrode.