Humana Press, Methods in Molecular Biology, p. 227-244, 2012
DOI: 10.1007/978-1-61779-539-8_15
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Over-expression is a valid functional genomics approach to characterise genes of unknown function on a genome-wide scale. Strains are engineered to over-express a specific gene and the resulting gain-of-function phenotype assessed. Here, we describe the strategy we are adopting to synthesise a Candida albicans ORFeome collection and the options available to create over-expressing strains from this collection.