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Oxford University Press, Nucleic Acids Research, 3(23), p. 464-466, 1995

DOI: 10.1093/nar/23.3.464

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A new mutation in 16S rRNA ofEscherichia coliconferring spectinomycin resistance

Journal article published in 1995 by Urban Johanson ORCID, Diarmaid Hughes ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We report a novel mutation, C1066U in 16S rRNA which was selected for resistance to spectinomycin, an antibiotic which inhibits ribosomal translocation. The minimal inhibitory concentration (MIC) of spectinomycin determined for this mutant (15 micrograms/ml) is greater than with the wild-type plasmid (5 micrograms/ml) but lower than with the well known C1192U mutation (> 80 micrograms/ml). The C1066U mutation also increases the cells sensitivity to fusidic acid, another antibiotic which inhibits translation at the translocation stage, whereas C1192U is unchanged relative to the wild type. We discuss why the acquisition of resistance to one of these drugs is often associated with hypersensitivity to the other.