Androgens are the key elements in the control of male reproductive function, exerting biological effects by interaction with their cognate intracellular receptors. The androgen receptor (AR), a member of the nuclear receptor superfamily, acts as a transcription factor modulating the gene-expression network in target tissues. Structurally, AR is composed of distinct functional domains: the amino-terminal domain (NTD encoded by exon 1), the DNA-binding domain (DBD encoded by exons 2 and 3), a hinge region, and the ligand-binding domain (LBD encoded by exons 4-8). The existence of alternatively spliced variants is well recognized for several members of the nuclear receptor superfamily. However, information on AR variants is mostly related to cancer and androgen insensitivity syndrome cases. Recently, some naturally occurring AR variants have been identified in healthy tissues of human and other vertebrate species, which awoke the interest in deciphering their role in non-pathological physiology. The objective of this chapter is to summarize the available data on natural alternatively spliced AR variants discussing how they could have a functional impact increasing the complexity of AR signaling.