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Indexing of viroids in citrus orchards of Campania, Southern Italy

Journal article published in 2005 by M. Malfitano, Patologia Vegetale, M. Barone ORCID, N. Duran Vila, D. Alioto
This paper is available in a repository.
This paper is available in a repository.

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Preprint: policy unknown
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Postprint: policy unknown
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Abstract

SUMMARY During the last three years a survey was made to eval- uate the presence and distribution of citrus viroids in the Campania region (Southern Italy). A total of 112 cit- rus trees of different species and cultivars were inspect- ed visually and sampled. One hundred and four out of the 112 samples graft-inoculated into Arizona 861-S1 "Etrog" citron gave typical viroid symptoms. Viroid in- fection was confirmed by sequential polyacrylamide gel electrophoresis (sPAGE) analysis and molecular hy- bridization of imprinted membranes. Hop stunt viroid (HSVd), Citrus viroid III (CVd-III) and Citrus exocortis viroid (CEVd) were the most widely detected, account- ing respectively for 87, 85 and 68% of the tested sources. Citrus viroid IV (CVd-IV) and Citrus bent leaf viroid (CBLVd), never reported in Italy before, were found in 24 and 13% of the sources. Most of the trees (82%) were infected with more than a viroid. The most frequent viroid combinations were CEVd + HSVd + CVd-III (43%), HSVd + CVd-III (17%) and CEVd + CBLVd + HSVd + CVd-III + CVd-IV (13%).